Aim —To evaluate the efficiency of phenol/chloroform, microwave, and Qiagen spin column based DNA extractions from paraffin wax embedded tissue for use in the polymerase chain reaction (PCR). In addition, to assess the reliability of amplifying a housekeeping gene to indicate successful viral DNA extraction. Methods —DNA samples extracted from 20 blocks of cervical carcinoma tissues using ...
Buffer PB (in Qiagen kit) Buffer PE (in Qiagen kit) Buffer EB (in Qiagen kit) QiaPrep spin column (1 per PCR reaction) 1.5 mL microcentrifuge tube (1 per PCR reaction) Procedure. Label spin columns and microcentrifuge tubes with the names of the appropriate PCR reactions
I did three 50µl reactions per library (plus water control), combined each library and column-purified using the QIAGEN MinElute PCR Purification kit (eluted in 50µl). Expected product size – 97bp. For some of the libraries I had to do 15 PCR cycles to get enough product. Digest: lab notebook 3: 15 and 26 june 2008
Apr 02, 2002 · QIAGEN offers a range of QIAquick and QIAEX Kits in single column, 8-well, and 96-well formats for specific cleanup applications. In 2000, QIAGEN launched a new range of cleanup kits, MinElute Kits, which use a new spin-column design developed at QIAGEN to allow elution of DNA fragments in a much lower volume than previously possible.
Place the QIAamp spin column in a clean 2 ml collection tube (provided), and discard the tube containing the filtrate. Do not over tighten caps. If caps are tightened until they snap they may loosen during centrifugation and subsequently damage the centrifuge. 8. Carefully open the QIAamp spin column and add 500µl Buffer AW1 without wetting ...
Bone powders are digested with proteinase K, and the DNA is purified directly using silica‐based spin columns (QIAquick™, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.
Tj recess tender